Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (2024)

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  2. Seahorse XFe24 Analyzers

Manufacturer Agilent Technologies

4.7

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5.0

|17 reviews

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XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.

Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (1)

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Seahorse XFe24 Analyzers

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Average Rating: 4.7

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17 Scientists have reviewed this product


5 out of 5
Ease of use

5 out of 5
After sales service

5 out of 5
Value for money

Write your own review



  • Arindam Dey
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (8)
  • Member since: 2023

  • Organization: BioMedX Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Promising results

Rating: 5.0


  • Application Area: Immunology

"Immunometabolism was always neglected before Seahorse XFe Analyzer. I used Seahorse XFe96 Analyzer and applied it in the field of immunotoxicology and showed in my research articles how the study of Immunometabolism of cells in presence of nanoparticles gets affected. In the future, I expect in drug discovery and toxicology studies seahorse XFe Analyzer will be one of the key instruments. Publications Published 1) Dey, A.K., et al., Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic Cells. Cells, 2021. 10(1). 2) Dey, A.K., et al., Tuning the Immunostimulation Properties of Cationic Lipid Nanocarriers for Nucleic Acid Delivery. Frontiers in Immunology 12."

Review date: 12 Feb 2023 | Seahorse XFe24 Analyzers

  • Rachel Newsome
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (12)
  • Member since: 2021

  • Organization: University of Florida



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5

Great post-purchase care

Rating: 4.0

  • Application Area:Analyze metabolic capacity of tumor cell lines

"The Agilent team gave an incredible introduction to the system and how to perform experiments following the purchase of our Ailent Seahorse xfe96, in addition to describing other applications that we had not yet considered. The consumable purchase plan is convenient as well."

Review date: 09 Dec 2021 | Seahorse XFe24 Analyzers

  • Ling Wang
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (16)
  • Member since: 2021

  • Organization: East Tennessee State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Overall, it is a very good equipment for analyzing mitochondrial metabolism!

Rating: 5.0

  • Application Area:Mitochondrial respiration

"It is easy to use. We got a lot of good data using the equipment, a few high-quality papers have been published."

Review date: 17 Nov 2021 | Seahorse XFe24 Analyzers

  • Jessica Pierce
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (20)
  • Member since: 2021

  • Organization: Emory University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5

Reliable, sensitive tool for cellular metabolism research!

Rating: 4.7

  • Application Area:Analyze glycolysis and mitochondrial respiration rates in cell monolayers

"The Seahorse XFe96 analyzer was a valuable tool for my research on BMSC cellular metabolism with aging. With the help of user guides and technical support, my mentor and I were able to optimize a protocol and generate useful data. Additionally, the 96-well capacity of this instrument and associated consumables allowed for large numbers of technical replicates with multiple experimental groups on the same plate. The machine and software were easy to use for protocol setup and group labeling, allowing for good record-keeping and data mining during the analysis steps. This is one of the best tools to have in cellular biology and metabolic research!"

Review date: 24 Sep 2021 | Seahorse XFe24 Analyzers

  • Prasanth Nadarajan
  • Status:

    Advanced Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (24)Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (25)
  • Member since: 2020

  • Organization: Biotech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Worth the price.

Rating: 5.0

  • Application Area:All aspects of cell analysis

"Excellent."

Review date: 19 Jun 2021 | Seahorse XFe24 Analyzers

  • Marina Macia
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (29)
  • Member since: 2020

  • Organization: University Of Salamanca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Great technology! It changed my life

Rating: 5.0

  • Application Area:Cell biology

"It is a game-changer in your metabolism assays, it helps you to better understand how mitochondria are working in your cells or tissues."

Review date: 09 Feb 2021 | Seahorse XFe24 Analyzers

  • Miriam Zamorano
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (33)
  • Member since: 2020

  • Organization: MD Anderson



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5

Great way to see the effect on cell faith and recovery after treatments.

Rating: 4.0

  • Application Area:Mitochondrial research

"Cellular metabolism is a critical factor in stem cell research with recent reports suggesting that both metabolic status and cell culture conditions can significantly alter the differentiation trajectory of stem cells, as well the effect of drugs like chemotherapy and the recovery of cells with stem cells treatment."

Review date: 09 Feb 2021 | Seahorse XFe24 Analyzers

  • Asad Ur Rehman
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (37)
  • Member since: 2018

  • Organization: JMI



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5

Must for cancer metabolism research.

Rating: 4.7

  • Application Area:Metabolism

"Must for metabolism experiment with clarity in real time."

Review date: 02 Feb 2021 | Seahorse XFe24 Analyzers

  • Muhammad Fayyaz
  • Status:

    Advanced Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (41)Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (42)
  • Member since: 2018

  • Organization: Univeristy of Illinois at Urbana-Champaign



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Great and precision results. A key to success for drug discovery.

Rating: 5.0

  • Application Area:Cell metabolism studies

"There are different models of the Agilent Seahorse available on the market, among them I found the best one is the Agilent Seahorse XFe96 Analyzer because the user can use 96 well plate means to measure the 96 samples in one run. We use the Agilent Seahorse XFe96 to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. The oxygen consumption rate (OCR) and extracellular acidification rates (ECAR) are key indicators of mitochondrial respiration and glycolysis as well as the ATP production rate. This measurement together provides a systems-level view of cellular metabolic function in cultured cells and in vitro samples. The story is not over here, one more powerful software tool is also available in the market like MITOsym from simulation plus. MITOsym uses for the mathematical modeling of the data obtained from the Seahorse XFe analyzer for cellular respiration and bioenergetics. The results obtained from the Seahorse XFe analyzer is very reliable and useful for the development and discovery of new drugs for cancer."

Review date: 14 Nov 2020 | Seahorse XFe24 Analyzers

  • Sweelin Chew
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (46)
  • Member since: 2019

  • Organization: University of Eastern Finland



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5

Intuitive instrument for live cell analysis.

Rating: 4.3

  • Application Area:Live cell analysis of mitochondrial function

"The Seahorse XFe96 cell analyzer is a benchtop instrument that is mainly intended for our group to analyze mitochondrial function in live cells. It is quite intuitive and the user interface is easy to use with simple directions to operate the analyzer. The data generated is medium throughput and will be adequate for a research group. However, given the cost of consumables from Agilent, researchers may be tempted to use certain reagents sold by other manufacturers."

Review date: 16 Mar 2020 | Seahorse XFe24 Analyzers



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  • Daniel Maloney
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (50)
  • Member since: 2019

  • Organization: Smurfit Institute of Genetics, Trinity College Dublin



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5

Fantastic method for examining mitochondrial health, but costs can add up quickly!

Rating: 4.0

  • Application Area:Analysing cell lines for mitochondrial deficits

"The Seahorse XFe96 is certainly the most powerful and effective tool I have used to measure oxygen consumption in my target cell populations. The plate format is a revelation compared to using the dual chamber Oxygraph (admittedly the Oxygraph has higher resolution, but the ability to examine 96 wells simultaneously is a real boon). The assays themselves are relatively straightforward to set up, but in my opinion, the software causes it to lose a star for Ease of Use - it is very slow to perform simple operations and this is even on the computer supplied with the machine. Furthermore, the data analyzer plug-ins can be very restrictive in what they allow you to do, such as defining where your acute injection was added in the scheme of the assay. All interactions I have had with technicians when looking for advice on assay set up etc has been top notch, they have been professional and helpful, so 5 stars for that. However, it is a very expensive machine to purchase and the consumables are no cheaper. It is possible of course to buy reagents from 3rd party retailers, but without the guarantee of them working, it can cost more in the long run. When you have a run that works well it is second to none in the amount of information you can gain, but when lengthy optimization processes are needed the costs can add up very quickly."

Review date: 06 Mar 2020 | Seahorse XFe24 Analyzers

  • Gloria Yang
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (54)
  • Member since: 2016

  • Organization: University of Southern California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Easy to use and good results.

Rating: 5.0

  • Application Area:Analyzing metabolic markers in cells

"Good data with the software - makes good graphic presentations."

Review date: 26 Nov 2019 | Seahorse XFe24 Analyzers

  • Ayesha Saleem
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (58)
  • Member since: 2016

  • Organization: Humber College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

An important instrument for any lab that measures metabolism.

Rating: 5.0

  • Application Area:Measure rates of glycolysis and oxygen consumption in isolated cells.

"The company provides you with excellent, thorough training and protocols before you start any experiments. As long as you follow the steps, the assay will work. Results are easily analyzed and quite reproducible. A very sensitive and effective tool to measure rates of glycolysis and oxygen consumption that you can monitor in real time."

Review date: 06 Nov 2017 | Seahorse XFe24 Analyzers

  • Min-Joon Han
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (62)
  • Member since: 2016

  • Organization: St. Jude



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5

It is great tool to check metabolism in the cells

Rating: 4.3

  • Application Area:stem cell metabolism

"It is not simple and cheap for all the users. But Seahorse also provide all the great consumables (plates, inhibitors, buffers) so it is really helpful for your experiment even you are the first user in your school/department."

Review date: 25 Jan 2017 | Seahorse XFe24 Analyzers

  • Jey Sabith Ebron
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (66)
  • Member since: 2016

  • Organization: Cleveland State University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5

Great results

Rating: 4.7

  • Application Area:Tumor energy metabolism

"Very sensitive data acquisition and processing. minimal error and excellent data "

Review date: 19 May 2016 | Seahorse XFe24 Analyzers

  • Elena Shevtsova
  • Status:

    Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (70)
  • Member since: 2009

  • Organization: IPAC RAS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5

Now Seahorse is the most useful instruments in our lab!

Rating: 4.7

  • Application Area:Analyse mitochondrial and cellular respiratory capacity, screening of mitoactive compounds

"Allow to provide significantly more experiments simultaneously, this is very important for isolated mitochondria as for screening potential drugs, as for analysing the bioenergetic pattern for brain mito after in vivo experiments "

Review date: 22 Jan 2016 | Seahorse XFe24 Analyzers

  • Henry Juguilon
  • Status:

    Advanced Reviewer Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (74)Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (75)
  • Member since: 2013

  • Organization: Howard Hughes Medical Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5

Rating: 5.0

  • Application Area:Cells

"It is so reliable that other labs in the institute are coming over to use it too."

Review date: 25 Mar 2015 | Seahorse XFe24 Analyzers

XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.

The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques. XFe Analyzers are available in two formats- 96-well for maximum throughput and flexibility, and 24-well for specialty samples such as islets and small model organisms.

Features:

  • Live-Cell, Real-Time Assays
  • Detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing
  • Direct Measure of Energy Metabolism
  • Validate results from protein/gene expression experiments with a functional assay
  • Label-free platform
  • No staining, analyte extraction, or destructive sample processing required
  • Minimal Sample Required
  • As few as 5000 cells/well (varies by cell type and instrument)
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    Breakthroughs in immunotherapy

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    Agilent Seahorse XF live-cell metabolism solutions for cancer research

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    Robust T cell activation with improved data quality and workflow

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (82)

    Real-time detection and modulation of human T cell activation

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (83)

    Characterization of Glycolysis with a Panel of Common Cellular Models Using Agilent Seahorse XF T...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (84)

    Bioenergetic Profiling of Cancer Cell Lines: Quantifying the Impact of Glycolysis on Cell Prolife...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (85)

    Changes In Metabolic Phenotype and Cellular ATP Production During CD4+ T Cell Activation

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (86)

    Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (87)

    Amyloid-β Binding Alcohol Dehydrogenase (ABAD), Estradiol and Mitochondrial Dysfunction in Alzhei...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (88)

    Interrogating Gynecological Cancer Cell Metabolisms at Different Oxygen Tensions: Results Using a...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (89)

    CaCo-2 and IPEC-J2 Cells have a Higher Fatty Acid Oxidation (FAO) Capacity than HuTu-80 or HepG2 ...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (90)

    Assessing Therapies for Traumatic Brain or Spinal Cord Injuries

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (91)

    Novel Tumor Model for the Characterization of Ovarian Cancer Spheroids

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (92)

    14-3-3ơ Suppresses Cancer Glycolysis, Glutaminolysis and Mitochondrial Biogenesis by Targeting c-...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (93)

    Age and High Fat Diet Associated Changes in Mitochondrial ROS Production in the Liver

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (94)

    Bioenergetics and Drug-Induced Toxicity in a Panel of Mouse Embryonic Fibroblasts with Mitochondr...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (95)

    Alterations in Mitochondrial Function, Neuropathology, and Memory in the 3xTg Model of Alzheimer’...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (96)

    Adenine and Pyridine Nucleotide Depletion by Nitric Oxide and Superoxide in Endothelial Cells: Im...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (97)

    Assessing Substrate Metabolism in Tumor Spheroids

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (98)

    Acute Insulin Resistance due to Insulin Receptor Antagonism Induces Dysregulation of Nuclear-Enco...

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (99)

    Normalizing XF Metabolic Data to Cellular or Mitochondrial Parameters

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (100)

    Metabolic Pathway Determination Using the XF24 Analyzer

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (101)

    Direct Comparison of Radiometric versus XF24 Flux Assays to Measure Substrate Utilization

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (102)

    Measuring the Metabolic Switch in Cancer Cells

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (103)

    Bioenergetic Analysis of Suspension Cells: Hematopoietic Stem Cells and Lymphocytes

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (104)

    Bioenergetic Analysis of Primary Neuronal Deficiencies

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (105)

    Defining Metabolic Phenotypes in Cancer Cells

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (106)

    Measuring glycolysis and oxidative metabolism in cancer cells

  • Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (107)

    Assessing mitochondrial dysfunction in primary cardiomyocytes

Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (108)

Editorial Article
Developing safe and effective serial killers of solid tumors
04 Aug 2022

Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (109)

Industry News
Insider insights on the strategy behind Agilent and BioTek’s combined live-cell analysis portfolio
27 Aug 2019

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Industry News
Integrating Metabolic Analysis and Imaging System for a Streamlined Experience
05 Apr 2018

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Industry News
Seahorse Bioscience Reaches 500 Scientific Paper Citations in 2014
13 Jan 2015

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Product News
UAB Develops a ‘Bioenergetic Health Index’ (BHI) Using Seahorse Bioscience XF Technology
30 May 2014

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    Agilent Seahorse XF live-cell metabolism solutions for cancer research

Product Overview

Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (114)

Seahorse XFe24 Analyzers

Manufacturer Agilent Technologies

4.7 / 5.0 | 17 reviews

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Promising results

Arindam Dey

BioMedX Institute

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Seahorse XFe24 Analyzers from Agilent Technologies | SelectScience (2024)

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